DKK3 抗体 (C-Term)
Quick Overview for DKK3 抗体 (C-Term) (ABIN374182)
抗原
See all DKK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-AAAALLGGEE I
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特异性
- This antibody detects Dickkopf-3 at C-term.
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3
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anti-DKK3 (AA 23-349) antibody
适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 91-350) antibody适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8A5C6 unconjugated
anti-DKK3 (AA 31-130) antibody适用: 小鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 91-350) antibody适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-DKK3 (AA 22-350) antibody适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DKK3 (AA 22-350) antibody适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DKK3 antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DKK3 antibody适用: 人, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4G7 unconjugated
anti-DKK3 antibody适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DKK3 antibody适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- DKK3
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别名
- Dickkopf-3 (DKK3)
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背景
- DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
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基因ID
- 27122
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NCBI登录号
- NP_037385
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UniProt
- Q9UBP4
抗原
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